› 4f18

X-ray diffraction
0.96Å resolution

Subatomic resolution structure of a high affinity periplasmic phosphate-binding protein (PfluDING) bound with arsenate at pH 8.5

Released:
DOI: 10.2210/pdb4f18/pdb
PDB entry 4f18 coloured by chain, front view.
PDB entry 4f18 coloured by chain, side view.
PDB entry 4f18 coloured by chain, top view.
Source organism: Pseudomonas fluorescens SBW25
Primary publication:
The molecular basis of phosphate discrimination in arsenate-rich environments.
Elias M, Wellner A, Goldin-Azulay K, Chabriere E, Vorholt JA, Erb TJ, Tawfik DS
Nature 491 134-7 (2012)
PMID: 23034649

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111161 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-binding protein PstS Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 39.12 KDa
Source organism: Pseudomonas fluorescens SBW25
Expression system: Escherichia coli
UniProt:
  • Canonical: C3K8K1 (Residues: 25-394; Coverage: 100%)
Gene name: PFLU_2427
Sequence domains: PBP superfamily domain
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand 8AR 1 x 8AR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 36.9Å b: 124.16Å c: 40.9Å
α: 90° β: 116.51° γ: 90°
R-values:
R R work R free
0.095 0.095 0.11
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Antimicrobial activity of metals: mechanisms, molecular targets and applications.
Lemire et al. (2013)
12 more

4 mentions without citation

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective.
Langton et al. (2016)
3 more