› 4f1r

X-ray diffraction
2.2Å resolution

Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginos

Released:
DOI: 10.2210/pdb4f1r/pdb
PDB entry 4f1r coloured by chain, front view.
PDB entry 4f1r coloured by chain, side view.
PDB entry 4f1r coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa.
Wei Y, Zhang H, Gao ZQ, Xu JH, Liu QS, Dong YH
IUBMB Life 65 50-7 (2013)
PMID: 23281037

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175925 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease/exonuclease/phosphatase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.61 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51380 (Residues: 1-259; Coverage: 100%)
Gene names: ALP65_01013, CAZ10_32350, DT376_09125, GNQ48_25180, GUL26_21635, IPC1295_10410, IPC737_07685, NCTC13621_05232, PAERUG_P19_London_7_VIM_2_05_10_01808, crc, exoA
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 74.299Å b: 74.299Å c: 123.575Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.246
Expression system: Escherichia coli

Quick links

Citations

1 review citation

sRNAs in bacterial type I and type III toxin-antitoxin systems.
Brantl et al. (2015)
2 other articles

1 mention without citation

The Pseudomonas aeruginosa catabolite repression control protein Crc is devoid of RNA binding activity.
Milojevic et al. (2013)