› 4f28

X-ray diffraction
1.55Å resolution

The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly

Released:
DOI: 10.2210/pdb4f28/pdb
PDB entry 4f28 coloured by chain, front view.
PDB entry 4f28 coloured by chain, side view.
PDB entry 4f28 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric control in a metalloprotein dramatically alters function.
Baxter EL, Zuris JA, Wang C, Vo PL, Axelrod HL, Cohen AE, Paddock ML, Nechushtai R, Onuchic JN, Jennings PA
Proc Natl Acad Sci U S A 110 948-53 (2013)
PMID: 23271805

Function and Biology Details

Reaction catalysed: 
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDGSH iron-sulfur domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NZ45 (Residues: 33-108; Coverage: 70%)
Gene names: C10orf70, CISD1, MDS029, ZCD1
Sequence domains: Iron-binding zinc finger CDGSH type
Structure domains: CDGSH iron-sulfur domain, mitoNEET-type

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 41.33Å b: 56.6Å c: 59.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

The unique fold and lability of the [2Fe-2S] clusters of NEET proteins mediate their key functions in health and disease.
Karmi et al. (2018)
12 other articles

3 mentions without citation

Allosteric control in a metalloprotein dramatically alters function.
Baxter et al. (2013)
2 more