› 4f2b

X-ray diffraction
2.16Å resolution

Modulation of S.Aureus Phosphatidylinositol-Specific Phospholipase C Membrane Binding

Released:
DOI: 10.2210/pdb4f2b/pdb
PDB entry 4f2b coloured by chain, front view.
PDB entry 4f2b coloured by chain, side view.
PDB entry 4f2b coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Primary publication:
Competition between anion binding and dimerization modulates Staphylococcus aureus phosphatidylinositol-specific phospholipase C enzymatic activity.
Cheng J, Goldstein R, Stec B, Gershenson A, Roberts MF
J Biol Chem 287 40317-27 (2012)
PMID: 23038258

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 35.24 KDa
Source organism: Staphylococcus aureus subsp. aureus str. Newman
Expression system: Escherichia coli
UniProt:
  • Canonical: P45723 (Residues: 11-312; Coverage: 100%)
Gene names: NWMN_0041, plc
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

1 bound ligand:
Ligand INS 2 x INS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 43.362Å b: 133.382Å c: 50.19Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.217 0.215 0.25
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Staphylococcus aureus Secreted Toxins and Extracellular Enzymes.
Tam et al. (2019)
7 other articles

1 mention without citation

Competition between anion binding and dimerization modulates Staphylococcus aureus phosphatidylinositol-specific phospholipase C enzymatic activity.
Cheng et al. (2012)