› 4f2e

X-ray diffraction
1.45Å resolution

Crystal structure of the Streptococcus pneumoniae D39 copper chaperone CupA with Cu(I)

Released:
DOI: 10.2210/pdb4f2e/pdb
PDB entry 4f2e coloured by chain, front view.
PDB entry 4f2e coloured by chain, side view.
PDB entry 4f2e coloured by chain, top view.
Source organism: Streptococcus pneumoniae D39
Primary publication:
A new structural paradigm in copper resistance in Streptococcus pneumoniae.
Fu Y, Tsui HC, Bruce KE, Sham LT, Higgins KA, Lisher JP, Kazmierczak KM, Maroney MJ, Dann CE, Winkler ME, Giedroc DP
Nat Chem Biol 9 177-83 (2013)
PMID: 23354287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EfeO-type cupredoxin-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 10.89 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2ZPL4 (Residues: 29-123; Coverage: 77%)
Gene name: SPD_0634
Sequence domains: Cupredoxin-like domain
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU1 2 x CU1
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 47.208Å b: 78.647Å c: 23.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Stress Physiology of Lactic Acid Bacteria.
Papadimitriou et al. (2016)
11 more

4 mentions without citation

Recent developments in copper and zinc homeostasis in bacterial pathogens.
Braymer et al. (2014)
3 more