› 4f2j

X-ray diffraction
2.64Å resolution

Crystal structure of ZNF217 bound to DNA, P6522 crystal form

Released:
DOI: 10.2210/pdb4f2j/pdb
PDB entry 4f2j coloured by chain, front view.
PDB entry 4f2j coloured by chain, side view.
PDB entry 4f2j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New insights into DNA recognition by zinc fingers revealed by structural analysis of the oncoprotein ZNF217.
Vandevenne M, Jacques DA, Artuz C, Nguyen CD, Kwan AH, Segal DJ, Matthews JM, Crossley M, Guss JM, Mackay JP
J Biol Chem 288 10616-27 (2013)
PMID: 23436653

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117444 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Zinc finger protein 217 Chain: C
Molecule details ›
Chain: C
Length: 57 amino acids
Theoretical weight: 6.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75362 (Residues: 469-523; Coverage: 5%)
Gene names: ZABC1, ZNF217
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*TP*TP*TP*GP*CP*AP*GP*AP*AP*TP*CP*GP*AP*TP*TP*CP*TP*GP*CP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF, null
Spacegroup: P6522
Unit cell:
a: 56.928Å b: 56.928Å c: 242.542Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.252 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

DNA methylation and de-methylation using hybrid site-targeting proteins.
Lei et al. (2018)
4 more

3 mentions without citation

New insights into DNA recognition by zinc fingers revealed by structural analysis of the oncoprotein ZNF217.
Vandevenne et al. (2013)
2 more