› 4f2m

X-ray diffraction
3Å resolution

Crystal structure of a TGEV coronavirus Spike fragment in complex with the TGEV neutralizing monoclonal antibody 1AF10

Released:
DOI: 10.2210/pdb4f2m/pdb
PDB entry 4f2m coloured by chain, front view.
PDB entry 4f2m coloured by chain, side view.
PDB entry 4f2m coloured by chain, top view.
Source organisms:
Primary publication:
Structural bases of coronavirus attachment to host aminopeptidase N and its inhibition by neutralizing antibodies.
Reguera J, Santiago C, Mudgal G, Ordoño D, Enjuanes L, Casasnovas JM
PLoS Pathog 8 e1002859 (2012)
PMID: 22876187

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211994 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
monoclonal antibody 1AF10, heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 221 amino acids
Theoretical weight: 23.64 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
monoclonal antibody 1AF10, light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 214 amino acids
Theoretical weight: 23.48 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Spike glycoprotein Chains: E, F
Molecule details ›
Chains: E, F
Length: 209 amino acids
Theoretical weight: 23.19 KDa
Source organism: TGEV virulent Purdue
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q0PKZ5 (Residues: 497-666; Coverage: 12%)
Gene name: S
Sequence domains: Coronavirus spike glycoprotein S1
Structure domains: Immunoglobulin-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand ACY 2 x ACY
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 82.77Å b: 106.05Å c: 103.52Å
α: 90° β: 95.13° γ: 90°
R-values:
R R work R free
0.218 0.216 0.251
Expression systems:
  • Not provided
  • Cricetulus griseus

Quick links

Citations

39 review citations

Coronavirus Spike Protein and Tropism Changes.
Hulswit et al. (2016)
38 more

8 mentions without citation

A structural view of coronavirus-receptor interactions.
Reguera et al. (2014)
7 more