› 4f3d

X-ray diffraction
2.5Å resolution

Structure of RPE65: P65 crystal form grown in Fos-Choline-10

Released:
DOI: 10.2210/pdb4f3d/pdb
PDB entry 4f3d coloured by chain, front view.
PDB entry 4f3d coloured by chain, side view.
PDB entry 4f3d coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis.
Kiser PD, Farquhar ER, Shi W, Sui X, Chance MR, Palczewski K
Proc Natl Acad Sci U S A 109 E2747-56 (2012)
PMID: 23012475

Function and Biology Details

Reactions catalysed: 
An all-trans-retinyl ester + H(2)O = 11-cis-retinol + a fatty acid
Lutein = meso-zeaxanthin
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinoid isomerohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 61.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q28175 (Residues: 1-533; Coverage: 100%)
Gene name: RPE65
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand ETX 3 x ETX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P65
Unit cell:
a: 175.258Å b: 175.258Å c: 86.449Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.219

Quick links

Citations

17 review citations

Chemistry of the retinoid (visual) cycle.
Kiser et al. (2014)
16 more

2 mentions without citation

Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis.
Kiser et al. (2012)
1 more