› 4f3m

X-ray diffraction
1.71Å resolution

Crystal structure of CRISPR-associated protein

Released:
DOI: 10.2210/pdb4f3m/pdb
PDB entry 4f3m coloured by chain, front view.
PDB entry 4f3m coloured by chain, side view.
PDB entry 4f3m coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
Cas5d protein processes pre-crRNA and assembles into a cascade-like interference complex in subtype I-C/Dvulg CRISPR-Cas system.
Nam KH, Haitjema C, Liu X, Ding F, Wang H, DeLisa MP, Ke A
Structure 20 1574-84 (2012)
PMID: 22841292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-191871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pre-crRNA processing endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.12 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KFY3 (Residues: 1-236; Coverage: 100%)
Gene name: BH0337
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, APS BEAMLINE 24-ID-C, APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 86.509Å b: 46.687Å c: 129.03Å
α: 90° β: 104.01° γ: 90°
R-values:
R R work R free
0.156 0.152 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

Genome editing. The new frontier of genome engineering with CRISPR-Cas9.
Doudna et al. (2014)
40 more

10 mentions without citation

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
9 more