› 4f3r

X-ray diffraction
2.25Å resolution

Structure of phosphopantetheine adenylyltransferase (CBU_0288) from Coxiella burnetii

Released:
DOI: 10.2210/pdb4f3r/pdb
PDB entry 4f3r coloured by chain, front view.
PDB entry 4f3r coloured by chain, side view.
PDB entry 4f3r coloured by chain, top view.
Source organism: Coxiella burnetii RSA 493
Primary publication:
Structural genomics for drug design against the pathogen Coxiella burnetii.
Franklin MC, Cheung J, Rudolph MJ, Burshteyn F, Cassidy M, Gary E, Hillerich B, Yao ZK, Carlier PR, Totrov M, Love JD
Proteins 83 2124-36 (2015)
PMID: 26033498

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-182943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 162 amino acids
Theoretical weight: 18.4 KDa
Source organism: Coxiella burnetii RSA 493
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83EM7 (Residues: 1-159; Coverage: 100%)
Gene names: CBU_0288, coaD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P321
Unit cell:
a: 103.965Å b: 103.965Å c: 89.862Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.21 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.
Martins et al. (2015)
3 more