› 4f3s

X-ray diffraction
2.14Å resolution

Crystal structure of periplasmic D-alanine ABC transporter from Salmonella enterica

Released:
DOI: 10.2210/pdb4f3s/pdb
PDB entry 4f3s coloured by chain, front view.
PDB entry 4f3s coloured by chain, side view.
PDB entry 4f3s coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Entry authors: Agarwal R, Chamala S, Evans B, Gizzi A, Hillerich B, Kar A, Lafleur J, Siedel R, Villigas G, Zencheck W, Foti R, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter D-alanine-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 29.6 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZPA3 (Residues: 23-253; Coverage: 100%)
Gene names: STM1633, dalS
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand GLY 1 x GLY
Ligand DAL 1 x DAL
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6122
Unit cell:
a: 80.406Å b: 80.406Å c: 187.041Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.221
Expression system: Escherichia coli BL21(DE3)

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