› 4f4c

X-ray diffraction
3.4Å resolution

The Crystal Structure of the Multi-Drug Transporter

Released:
DOI: 10.2210/pdb4f4c/pdb
PDB entry 4f4c coloured by chain, front view.
PDB entry 4f4c coloured by chain, side view.
PDB entry 4f4c coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure of the multidrug transporter P-glycoprotein from Caenorhabditis elegans.
Jin MS, Oldham ML, Zhang Q, Chen J
Nature 490 566-9 (2012)
PMID: 23000902

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Multidrug resistance protein pgp-1 Chain: A
Molecule details ›
Chain: A
Length: 1321 amino acids
Theoretical weight: 145.22 KDa
Source organism: Caenorhabditis elegans
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P34712 (Residues: 1-1321; Coverage: 100%)
Gene names: K08E7.9, pgp-1
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand 0SA 2 x 0SA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 96.896Å b: 155.36Å c: 162.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.283
Expression system: Komagataella pastoris

Quick links

Citations

58 review citations

Mechanistic diversity in ATP-binding cassette (ABC) transporters.
Locher KP. (2016)
57 more

52 mentions without citation

Structural diversity of ABC transporters.
ter Beek et al. (2014)
51 more