› 4f61

X-ray diffraction
4.17Å resolution

Tubulin:Stathmin-like domain complex

Released:
DOI: 10.2210/pdb4f61/pdb
PDB entry 4f61 coloured by chain, front view.
PDB entry 4f61 coloured by chain, side view.
PDB entry 4f61 coloured by chain, top view.
Source organisms:
Primary publication:
Design and characterization of modular scaffolds for tubulin assembly.
Mignot I, Pecqueur L, Dorléans A, Karuppasamy M, Ravelli RB, Dreier B, Plückthun A, Knossow M, Gigant B
J Biol Chem 287 31085-94 (2012)
PMID: 22791712

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-112172 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 445 amino acids
Theoretical weight: 49.98 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Stathmin-like domain R4 Chain: I
Molecule details ›
Chain: I
Length: 240 amino acids
Theoretical weight: 28.78 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand GTP 4 x GTP
Ligand MG 4 x MG
Ligand GDP 4 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 639.74Å b: 66.1Å c: 128.13Å
α: 90° β: 92.02° γ: 90°
R-values:
R R work R free
0.242 0.241 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies.
Knossow et al. (2020)
1 more

2 mentions without citation

Design and characterization of modular scaffolds for tubulin assembly.
Mignot et al. (2012)
1 more