› 4f62

X-ray diffraction
2.1Å resolution

Crystal structure of a putative farnesyl-diphosphate synthase from Marinomonas sp. MED121 (Target EFI-501980)

Released:
DOI: 10.2210/pdb4f62/pdb
PDB entry 4f62 coloured by chain, front view.
PDB entry 4f62 coloured by chain, side view.
PDB entry 4f62 coloured by chain, top view.
Source organism: Marinomonas sp. MED121
Primary publication:
Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD
Proc Natl Acad Sci U S A 110 E1196-202 (2013)
PMID: 23493556

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranyltranstransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.1 KDa
Source organism: Marinomonas sp. MED121
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3YCB7 (Residues: 1-293; Coverage: 100%)
Gene name: MED121_18165
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 96.491Å b: 96.491Å c: 156.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
14 more

1 mention without citation

Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp et al. (2013)