› 4f6e

X-ray diffraction
1.6Å resolution

Crystal Structure of the K182R, A183P mutant manganese superoxide dismutase from Sacchromyces cerevisiae

Released:
DOI: 10.2210/pdb4f6e/pdb
PDB entry 4f6e coloured by chain, front view.
PDB entry 4f6e coloured by chain, side view.
PDB entry 4f6e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Sheng Y, Cascio D, Valentine JS

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn], mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 23.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00447 (Residues: 27-233; Coverage: 89%)
Gene names: SOD2, YHR008C
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand TRS 1 x TRS
Ligand GOL 1 x GOL
1 modified residue:
Ligand MLY 60 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 65.54Å b: 66.25Å c: 66.62Å
α: 112.58° β: 103.63° γ: 110.27°
R-values:
R R work R free
0.166 0.164 0.195
Expression system: Saccharomyces cerevisiae

Quick links