› 4f6r

X-ray diffraction
2.64Å resolution

Tubulin:Stathmin-like domain complex

Released:
DOI: 10.2210/pdb4f6r/pdb
PDB entry 4f6r coloured by chain, front view.
PDB entry 4f6r coloured by chain, side view.
PDB entry 4f6r coloured by chain, top view.
Source organisms:
Primary publication:
Design and characterization of modular scaffolds for tubulin assembly.
Mignot I, Pecqueur L, Dorléans A, Karuppasamy M, Ravelli RB, Dreier B, Plückthun A, Knossow M, Gigant B
J Biol Chem 287 31085-94 (2012)
PMID: 22791712

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-112162 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50.04 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Stathmin-like domain R1 Chain: C
Molecule details ›
Chain: C
Length: 87 amino acids
Theoretical weight: 10.12 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Designed ankyrin repeat protein (DARPIN) D2 Chain: D
Molecule details ›
Chain: D
Length: 169 amino acids
Theoretical weight: 18.14 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

5 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand GDP 1 x GDP
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 95.06Å b: 75.57Å c: 155.68Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.178 0.177 0.201
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

2 review citations

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies.
Knossow et al. (2020)
1 more

6 mentions without citation

TRPC6 G757D Loss-of-Function Mutation Associates with FSGS.
Riehle et al. (2016)
5 more