› 4f79

X-ray diffraction
2.54Å resolution

The crystal structure of 6-phospho-beta-glucosidase mutant (E375Q) in complex with Salicin 6-phosphate

Released:
DOI: 10.2210/pdb4f79/pdb
Model geometry
Fit model/data
PDB entry 4f79 coloured by chain, front view.
PDB entry 4f79 coloured by chain, side view.
PDB entry 4f79 coloured by chain, top view.
Source organism: Streptococcus mutans
Primary publication:
GH1-family 6-P-β-glucosidases from human microbiome lactic acid bacteria.
Michalska K, Tan K, Li H, Hatzos-Skintges C, Bearden J, Babnigg G, Joachimiak A
OpenAccess logo Acta Crystallogr D Biol Crystallogr 69 451-63 (2013)
PMID: 23519420

Function and Biology Details

Reaction catalysed: 
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 55.47 KDa
Source organism: Streptococcus mutans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DT00 (Residues: 1-477; Coverage: 100%)
Gene names: SMU_1601, bgl
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand P53 1 x P53
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 82.278Å b: 82.278Å c: 221.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

β-Glucosidase activities of lactic acid bacteria: mechanisms, impact on fermented food and human health.
Michlmayr et al. (2014)
1 more

3 mentions without citation

Structural insights into the substrate specificity of a 6-phospho-β-glucosidase BglA-2 from Streptococcus pneumoniae TIGR4.
Yu et al. (2013)
2 more