› 4f7r

X-ray diffraction
3.2Å resolution

Crystal structure of 14-3-3 protein from Giardia intestinalis

Released:
DOI: 10.2210/pdb4f7r/pdb
PDB entry 4f7r coloured by chain, front view.
PDB entry 4f7r coloured by chain, side view.
PDB entry 4f7r coloured by chain, top view.
Source organism: Giardia intestinalis
Primary publication:
The crystal structure of Giardia duodenalis 14-3-3 in the apo form: when protein post-translational modifications make the difference.
Fiorillo A, di Marino D, Bertuccini L, Via A, Pozio E, Camerini S, Ilari A, Lalle M
PLoS One 9 e92902 (2014)
PMID: 24658679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14-3-3 protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 253 amino acids
Theoretical weight: 29.02 KDa
Source organism: Giardia intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: E2RU97 (Residues: 1-248; Coverage: 100%)
Gene names: GL50803_006430, GL50803_6430
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P32
Unit cell:
a: 100.879Å b: 100.879Å c: 140.559Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Modulators of 14-3-3 Protein-Protein Interactions.
Stevers et al. (2018)
2 more

1 mention without citation

The crystal structure of Giardia duodenalis 14-3-3 in the apo form: when protein post-translational modifications make the difference.
Fiorillo et al. (2014)