› 4f92

X-ray diffraction
2.66Å resolution

Brr2 Helicase Region S1087L

Released:
DOI: 10.2210/pdb4f92/pdb
PDB entry 4f92 coloured by chain, front view.
PDB entry 4f92 coloured by chain, side view.
PDB entry 4f92 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome.
Santos KF, Jovin SM, Weber G, Pena V, Lührmann R, Wahl MC
Proc Natl Acad Sci U S A 109 17418-23 (2012)
PMID: 23045696

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U5 small nuclear ribonucleoprotein 200 kDa helicase Chain: B
Molecule details ›
Chain: B
Length: 1724 amino acids
Theoretical weight: 197.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75643 (Residues: 402-2125; Coverage: 81%)
Gene names: ASCC3L1, BRR2, HELIC2, KIAA0788, SNRNP200
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAN 1 x SAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 146.148Å b: 149.541Å c: 141.324Å
α: 90° β: 120.33° γ: 90°
R-values:
R R work R free
0.214 0.212 0.259
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Mechanistic insights into precursor messenger RNA splicing by the spliceosome.
Shi Y. (2017)
14 more

3 mentions without citation

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.
Hochstrasser et al. (2014)
2 more