› 4fay

X-ray diffraction
1.56Å resolution

Crystal structure of a trimeric bacterial microcompartment shell protein PduB with glycerol metabolites

Released:
DOI: 10.2210/pdb4fay/pdb
PDB entry 4fay coloured by chain, front view.
PDB entry 4fay coloured by chain, side view.
PDB entry 4fay coloured by chain, top view.
Source organism: Limosilactobacillus reuteri subsp. reuteri
Primary publication:
Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB.
Pang A, Liang M, Prentice MB, Pickersgill RW
Acta Crystallogr D Biol Crystallogr 68 1642-52 (2012)
PMID: 23151629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial microcompartment shell protein PduB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 258 amino acids
Theoretical weight: 27.05 KDa
Source organism: Limosilactobacillus reuteri subsp. reuteri
Expression system: Escherichia coli
UniProt:
  • Canonical: A5VMB3 (Residues: 1-238; Coverage: 100%)
Gene names: Lreu_1748, pduB
Sequence domains: BMC domain

Ligands and Environments

2 bound ligands:
Ligand GOL 17 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 69.865Å b: 120.864Å c: 145.531Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.202
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Bacterial microcompartments.
Kerfeld et al. (2018)
8 more

7 mentions without citation

Recent structural insights into bacterial microcompartment shells.
Ochoa et al. (2021)
6 more