› 4fb0

X-ray diffraction
3.22Å resolution

Structure of Oceanobacillus iheyensis group II intron C377G mutant in a ligand-free state in the presence of K+ and Mg2+

Released:
DOI: 10.2210/pdb4fb0/pdb
PDB entry 4fb0 coloured by chain, front view.
PDB entry 4fb0 coloured by chain, side view.
PDB entry 4fb0 coloured by chain, top view.
Source organism: Oceanobacillus iheyensis
Primary publication:
Visualizing group II intron catalysis through the stages of splicing.
Marcia M, Pyle AM
Cell 151 497-507 (2012)
PMID: 23101623

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-195918 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Group IIC intron Chain: A
Molecule details ›
Chain: A
Length: 393 nucleotides
Theoretical weight: 127.69 KDa
Source organism: Oceanobacillus iheyensis
Expression system: Not provided
Sequence domains:
  • Group II catalytic intron D1-D4-3
  • Group II catalytic intron D1-D4-1

    • Ligands and Environments

    4 bound ligands:
    Ligand MG 25 x MG
    Ligand K 18 x K
    Ligand EPE 5 x EPE
    Ligand SPM 1 x SPM
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    X-ray source: APS BEAMLINE 24-ID-C
    Spacegroup: P212121
    Unit cell:
    a: 89.188Å b: 95.444Å c: 224.706Å
    α: 90° β: 90° γ: 90°
    R-values:
    R R work R free
    0.186 0.184 0.23
    Expression system: Not provided

    Quick links

    Citations

    29 review citations

    Mechanisms and Regulation of Alternative Pre-mRNA Splicing.
    Lee et al. (2015)
    28 more

    4 mentions without citation

    Visualizing group II intron catalysis through the stages of splicing.
    Marcia et al. (2012)
    3 more