› 4fbl

X-ray diffraction
1.99Å resolution

LipS and LipT, two metagenome-derived lipolytic enzymes increase the diversity of known lipase and esterase families

Released:
DOI: 10.2210/pdb4fbl/pdb
PDB entry 4fbl coloured by chain, front view.
PDB entry 4fbl coloured by chain, side view.
PDB entry 4fbl coloured by chain, top view.
Source organism: unidentified
Primary publication:
The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases.
Chow J, Kovacic F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C, Lauinger B, Bongen P, Pietruszka J, Schmidt M, Menyes I, Bornscheuer UT, Eckstein M, Thum O, Liese A, Mueller-Dieckmann J, Jaeger KE, Streit WR
PLoS One 7 e47665 (2012)
PMID: 23112831

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LipS lipolytic enzyme Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 281 amino acids
Theoretical weight: 30.27 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand SPD 3 x SPD
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P4
Unit cell:
a: 105.27Å b: 105.27Å c: 120.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Immobilised lipases in the cosmetics industry.
Ansorge-Schumacher et al. (2013)
4 more

2 mentions without citation

The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases.
Chow et al. (2012)
1 more