› 4fe5

X-ray diffraction
1.32Å resolution

Crystal structure of the xpt-pbuX guanine riboswitch aptamer domain in complex with hypoxanthine

Released:
DOI: 10.2210/pdb4fe5/pdb
PDB entry 4fe5 coloured by chain, front view.
PDB entry 4fe5 coloured by chain, side view.
PDB entry 4fe5 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine.
Batey RT, Gilbert SD, Montange RK
Nature 432 411-5 (2004)
PMID: 15549109

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196801 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
xpt-pbuX guanine riboswitch aptamer domain Chain: B
Molecule details ›
Chain: B
Length: 67 nucleotides
Theoretical weight: 21.51 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
Sequence domains: Purine riboswitch

Ligands and Environments

3 bound ligands:
Ligand HPA 1 x HPA
Ligand ACT 2 x ACT
Ligand NCO 6 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 133.247Å b: 35.25Å c: 41.833Å
α: 90° β: 90.75° γ: 90°
R-values:
R R work R free
0.196 0.196 0.229
Expression system: Not provided

Quick links

Citations

78 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
77 more

24 mentions without citation

Long-Range Interactions in Riboswitch Control of Gene Expression.
Jones et al. (2017)
23 more