› 4fek

X-ray diffraction
2Å resolution

Crystal Structure of putative diflavin flavoprotein A 5 (fragment 1-254) from Nostoc sp. PCC 7120, Northeast Structural Genomics Consortium Target NsR435A , Northeast Structural Genomics Consortium (NESG) Target NsR435A

Released:
DOI: 10.2210/pdb4fek/pdb
PDB entry 4fek coloured by chain, front view.
PDB entry 4fek coloured by chain, side view.
PDB entry 4fek coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Kuzin A, Abashidze M, Seetharaman J, Janjua J, Xiao R, Foote EL, Ciccosanti C, Wang H, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative diflavin flavoprotein A 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.96 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Z0C1 (Residues: 1-254; Coverage: 45%)
Gene names: all0177, dfa5
Sequence domains: ODP family beta lactamase
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand PEG 2 x PEG
Ligand CL 1 x CL
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 50.651Å b: 57.993Å c: 106.606Å
α: 90° β: 102.42° γ: 90°
R-values:
R R work R free
0.211 0.209 0.24
Expression system: Escherichia coli BL21(DE3)

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