› 4fez

X-ray diffraction
2.16Å resolution

Inosine 5'-monophosphate dehydrogenase from Vibrio cholerae, deletion mutant

Released:
DOI: 10.2210/pdb4fez/pdb
PDB entry 4fez coloured by chain, front view.
PDB entry 4fez coloured by chain, side view.
PDB entry 4fez coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Osipiuk J, Maltseva N, Makowska-Grzyska M, Gu M, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-192099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 40.77 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KTW3 (Residues: 1-489; Coverage: 79%)
Gene names: VC_0767, guaB
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand PEG 2 x PEG
Ligand EDO 2 x EDO
Ligand FMT 6 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P4212
Unit cell:
a: 110.005Å b: 110.005Å c: 166.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.193
Expression system: Escherichia coli

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