› 4ff7

X-ray diffraction
1.86Å resolution

Structure of C126S mutant of Saccharomyces cerevisiae triosephosphate isomerase

Released:
DOI: 10.2210/pdb4ff7/pdb
PDB entry 4ff7 coloured by chain, front view.
PDB entry 4ff7 coloured by chain, side view.
PDB entry 4ff7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Effects of a buried cysteine-to-serine mutation on yeast triosephosphate isomerase structure and stability.
Hernández-Santoyo A, Domínguez-Ramírez L, Reyes-López CA, González-Mondragón E, Hernández-Arana A, Rodríguez-Romero A
Int J Mol Sci 13 10010-10021 (2012)
PMID: 22949845

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00942 (Residues: 1-248; Coverage: 100%)
Gene names: TPI1, YD9609.05C, YDR050C
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand PGA 1 x PGA
Ligand GOL 12 x GOL
Ligand NA 1 x NA
Ligand SO4 2 x SO4
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 46.923Å b: 61.439Å c: 160.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.176 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Cytosolic Triosephosphate Isomerase from Arabidopsis thaliana Is Reversibly Modified by Glutathione on Cysteines 127 and 218.
Dumont et al. (2016)
4 more

2 mentions without citation

PDB-Explorer: a web-based interactive map of the protein data bank in shape space.
Jin et al. (2015)
1 more