› 4ffw

X-ray diffraction
2.9Å resolution

Crystal Structure of Dipeptidyl Peptidase IV (DPP4, DPP-IV, CD26) in Complex with Fab + sitagliptin

Released:
DOI: 10.2210/pdb4ffw/pdb
PDB entry 4ffw coloured by chain, front view.
PDB entry 4ffw coloured by chain, side view.
PDB entry 4ffw coloured by chain, top view.
Source organisms:
Entry authors: Wang Z, Sudom A, Walker NP, Min X

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210606 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Dipeptidyl peptidase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 730 amino acids
Theoretical weight: 84.34 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
UniProt:
  • Canonical: P14740 (Residues: 38-767; Coverage: 95%)
Gene names: Cd26, Dpp4
Sequence domains:
Structure domains:
Fab light chain Chains: C, L
Molecule details ›
Chains: C, L
Length: 210 amino acids
Theoretical weight: 22.9 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab heavy chain Chains: D, H
Molecule details ›
Chains: D, H
Length: 217 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand 715 2 x 715
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 97.77Å b: 200.86Å c: 97.93Å
α: 90° β: 93.64° γ: 90°
R-values:
R R work R free
0.288 0.287 0.311
Expression system: Homo sapiens

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