4fgn

X-ray diffraction
3.2Å resolution

Crystal structure of the SV40 large T-antigen origin bining domain bound to Site I DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117479 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.24 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03070 (Residues: 131-260; Coverage: 18%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,
Site I DNA Chain: Z
Molecule details ›
Chain: Z
Length: 23 nucleotides
Theoretical weight: 6.96 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Not provided
Site I DNA Chain: Y
Molecule details ›
Chain: Y
Length: 23 nucleotides
Theoretical weight: 7.16 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 58.604Å b: 139.672Å c: 132.112Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.267
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided