› 4fh0

X-ray diffraction
1.4Å resolution

Crystal Structure of Human BinCARD CARD, double mutant F16M/L66M SeMet form

Released:
DOI: 10.2210/pdb4fh0/pdb
PDB entry 4fh0 coloured by chain, front view.
PDB entry 4fh0 coloured by chain, side view.
PDB entry 4fh0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the caspase recruitment domain of BinCARD reveals that all three cysteines can be oxidized.
Chen KE, Richards AA, Caradoc-Davies TT, Vajjhala PR, Robin G, Lua LH, Hill JM, Schroder K, Sweet MJ, Kellie S, Kobe B, Martin J
Acta Crystallogr D Biol Crystallogr 69 774-84 (2013)
PMID: 23633586

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase recruitment domain-containing protein 19 Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 12.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96LW7 (Residues: 3-101; Coverage: 43%)
Gene names: C9orf89, CARD19
Structure domains: Death Domain, Fas

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 32.196Å b: 36.669Å c: 43.276Å
α: 108.54° β: 90.3° γ: 111.01°
R-values:
R R work R free
0.14 0.137 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The versatile roles of CARDs in regulating apoptosis, inflammation, and NF-κB signaling.
Kao et al. (2015)
7 other articles

2 mentions without citation

Structure of the caspase-recruitment domain from a zebrafish guanylate-binding protein.
Jin et al. (2013)
1 more