› 4fju

X-ray diffraction
1.77Å resolution

Crystal structure of ureidoglycolate dehydrogenase in ternary complex with NADH and glyoxylate

Released:
DOI: 10.2210/pdb4fju/pdb
PDB entry 4fju coloured by chain, front view.
PDB entry 4fju coloured by chain, side view.
PDB entry 4fju coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. DH10B
Primary publication:
Structural and functional insights into (S)-ureidoglycolate dehydrogenase, a metabolic branch point enzyme in nitrogen utilization.
Kim MI, Shin I, Cho S, Lee J, Rhee S
PLoS One 7 e52066 (2012)
PMID: 23284870

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ureidoglycolate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 351 amino acids
Theoretical weight: 38.2 KDa
Source organism: Escherichia coli str. K-12 substr. DH10B
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAI 2 x NAI
Ligand GLV 1 x GLV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P42212
Unit cell:
a: 162.756Å b: 162.756Å c: 61.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Ureidoglycolate hydrolase, amidohydrolase, lyase: how errors in biological databases are incorporated in scientific papers and vice versa.
Percudani et al. (2013)
2 more

1 mention without citation

Structural and functional insights into (S)-ureidoglycolate dehydrogenase, a metabolic branch point enzyme in nitrogen utilization.
Kim et al. (2012)