› 4fkb

X-ray diffraction
1.22Å resolution

An Organic solvent tolerant lipase 42

Released:
DOI: 10.2210/pdb4fkb/pdb
PDB entry 4fkb coloured by chain, front view.
PDB entry 4fkb coloured by chain, side view.
PDB entry 4fkb coloured by chain, top view.
Source organism: Bacillus sp. 42
Entry authors: Zaliha RN, Rahman RA, Khusaini MS

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.29 KDa
Source organism: Bacillus sp. 42
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5U780 (Residues: 29-416; Coverage: 100%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 21 x GOL
Ligand NA 9 x NA
Ligand CL 10 x CL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 117.824Å b: 81.001Å c: 99.529Å
α: 90° β: 96.69° γ: 90°
R-values:
R R work R free
0.129 0.127 0.165
Expression system: Escherichia coli

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