› 4fl6

X-ray diffraction
2.55Å resolution

Crystal structure of the complex of the 3-MBT repeat domain of L3MBTL3 and UNC1215

Released:
DOI: 10.2210/pdb4fl6/pdb
PDB entry 4fl6 coloured by chain, front view.
PDB entry 4fl6 coloured by chain, side view.
PDB entry 4fl6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of a chemical probe for the L3MBTL3 methyllysine reader domain.
James LI, Barsyte-Lovejoy D, Zhong N, Krichevsky L, Korboukh VK, Herold JM, MacNevin CJ, Norris JL, Sagum CA, Tempel W, Marcon E, Guo H, Gao C, Huang XP, Duan S, Emili A, Greenblatt JF, Kireev DB, Jin J, Janzen WP, Brown PJ, Bedford MT, Arrowsmith CH, Frye SV
Nat Chem Biol 9 184-91 (2013)
PMID: 23292653

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lethal(3)malignant brain tumor-like protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 38.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96JM7 (Residues: 229-553; Coverage: 42%)
Gene names: KIAA1798, L3MBTL3, MBT1
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.

Ligands and Environments

2 bound ligands:
Ligand UWN 2 x UWN
Ligand UNX 29 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 228.026Å b: 54.372Å c: 65.549Å
α: 90° β: 97.11° γ: 90°
R-values:
R R work R free
0.198 0.195 0.259
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Chromatin proteins and modifications as drug targets.
Helin et al. (2013)
36 more

7 mentions without citation

Towards understanding methyllysine readout.
Musselman et al. (2014)
6 more