Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero pentamer (preferred)
Assembly name:
Protease (preferred)
PDBe Complex ID:
PDB-CPX-136855 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protease
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.73 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases
Length: 99 amino acids
Theoretical weight: 10.73 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P03367 (Residues: 501-599; Coverage: 7%)
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases
Protease
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 488 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 4 amino acids
Theoretical weight: 488 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P03367 (Residues: 560-563; Coverage: 0%)
p15
Molecule details ›
Chain: E
Length: 3 amino acids
Theoretical weight: 372 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 3 amino acids
Theoretical weight: 372 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P03367 (Residues: 1119-1121; Coverage: 0%)
Protease
Molecule details ›
Chain: F
Length: 3 amino acids
Theoretical weight: 373 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 3 amino acids
Theoretical weight: 373 Da
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P03367 (Residues: 564-566; Coverage: 0%)
