› 4fma

X-ray diffraction
2.15Å resolution

EspG structure

Released:
DOI: 10.2210/pdb4fma/pdb
PDB entry 4fma coloured by chain, front view.
PDB entry 4fma coloured by chain, side view.
PDB entry 4fma coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structurally distinct bacterial TBC-like GAPs link Arf GTPase to Rab1 inactivation to counteract host defenses.
Dong N, Zhu Y, Lu Q, Hu L, Zheng Y, Shao F
Cell 150 1029-41 (2012)
PMID: 22939626

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EspG protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 351 amino acids
Theoretical weight: 38.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5WMC0 (Residues: 47-397; Coverage: 88%)
Gene name: espG
Sequence domains: EspG protein
Structure domains: EspG protein, N-terminal domain

Ligands and Environments

3 bound ligands:
Ligand MG 19 x MG
Ligand FMT 6 x FMT
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 94.615Å b: 167.739Å c: 361.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

65 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
64 more

2 mentions without citation

Solvent-modulation of the structure and dimensionality in lanthanoid-anilato coordination polymers.
Benmansour et al. (2018)
1 more