› 4fmp

X-ray diffraction
2.3Å resolution

Crystal structure of thermostable, organic-solvent tolerant lipase from Geobacillus sp. strain ARM

Released:
DOI: 10.2210/pdb4fmp/pdb
PDB entry 4fmp coloured by chain, front view.
PDB entry 4fmp coloured by chain, side view.
PDB entry 4fmp coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystallization of novel ARM lipase and elucidation of its space-grown crystal structure
Nisbar ND, Rahman RNZRA, Ali MSM, Leow ATC
Thesis (2013)

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 42.55 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93A71 (Residues: 35-415; Coverage: 91%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 55.786Å b: 143.392Å c: 63.965Å
α: 90° β: 105.88° γ: 90°
R-values:
R R work R free
0.176 0.173 0.242
Expression system: Escherichia coli

Quick links