› 4fmv

X-ray diffraction
2.01Å resolution

Crystal Structure Analysis of a GH30 Endoxylanase from Clostridium papyrosolvens C71

Released:
DOI: 10.2210/pdb4fmv/pdb
PDB entry 4fmv coloured by chain, front view.
PDB entry 4fmv coloured by chain, side view.
PDB entry 4fmv coloured by chain, top view.
Source organism: Ruminiclostridium papyrosolvens DSM 2782
Primary publication:
A novel member of glycoside hydrolase family 30 subfamily 8 with altered substrate specificity.
St John FJ, Dietrich D, Crooks C, Pozharski E, González JM, Bales E, Smith K, Hurlbert JC
Acta Crystallogr D Biol Crystallogr 70 2950-8 (2014)
PMID: 25372685

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.94 KDa
Source organism: Ruminiclostridium papyrosolvens DSM 2782
Expression system: Escherichia coli
UniProt:
  • Canonical: F1TBY8 (Residues: 34-419; Coverage: 64%)
Gene name: Cpap_2855
Sequence domains: Glycosyl hydrolase family 59
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 66.02Å b: 76.52Å c: 150.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.2
Expression system: Escherichia coli

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Citations

3 review citations

Endo-xylanases as tools for production of substituted xylooligosaccharides with prebiotic properties.
Nordberg Karlsson et al. (2018)
2 more

6 mentions without citation

Structural analysis of glucuronoxylan-specific Xyn30D and its attached CBM35 domain gives insights into the role of modularity in specificity.
Sainz-Polo et al. (2014)
5 more