› 4fng

X-ray diffraction
1.95Å resolution

The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina

Released:
DOI: 10.2210/pdb4fng/pdb
PDB entry 4fng coloured by chain, front view.
PDB entry 4fng coloured by chain, side view.
PDB entry 4fng coloured by chain, top view.
Source organism: Lucilia cuprina
Primary publication:
Structure and function of an insect α-carboxylesterase (αEsterase7) associated with insecticide resistance.
Jackson CJ, Liu JW, Carr PD, Younus F, Coppin C, Meirelles T, Lethier M, Pandey G, Ollis DL, Russell RJ, Weik M, Oakeshott JG
Proc Natl Acad Sci U S A 110 10177-82 (2013)
PMID: 23733941

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-173217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic ester hydrolase Chain: A
Molecule details ›
Chain: A
Length: 570 amino acids
Theoretical weight: 65.47 KDa
Source organism: Lucilia cuprina
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q25252 (Residues: 1-570; Coverage: 100%)
Gene name: LcaE7
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 48.62Å b: 100.51Å c: 221.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Genotype to phenotype, the molecular and physiological dimensions of resistance in arthropods.
Feyereisen et al. (2015)
5 more

4 mentions without citation

Dynamic Roles of Insect Carboxyl/Cholinesterases in Chemical Adaptation.
Cruse et al. (2023)
3 more