› 4fnz

X-ray diffraction
2.6Å resolution

Crystal structure of human anaplastic lymphoma kinase in complex with piperidine-carboxamide inhibitor 2

Released:
DOI: 10.2210/pdb4fnz/pdb
PDB entry 4fnz coloured by chain, front view.
PDB entry 4fnz coloured by chain, side view.
PDB entry 4fnz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The R1275Q neuroblastoma mutant and certain ATP-competitive inhibitors stabilize alternative activation loop conformations of anaplastic lymphoma kinase.
Epstein LF, Chen H, Emkey R, Whittington DA
J Biol Chem 287 37447-57 (2012)
PMID: 22932897

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ALK tyrosine kinase receptor Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.83 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UM73 (Residues: 1084-1410; Coverage: 20%)
Gene name: ALK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NZF 1 x NZF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 51.496Å b: 58.059Å c: 105.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.198 0.261
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Mechanistic insight into ALK receptor tyrosine kinase in human cancer biology.
Hallberg et al. (2013)
5 more

4 mentions without citation

ALK and ROS1 as a joint target for the treatment of lung cancer: a review.
Puig de la Bellacasa et al. (2013)
3 more