› 4fof

X-ray diffraction
2.42Å resolution

Crystal Structure of the blue-light absorbing form of the Thermosynechococcus elongatus PixJ GAF-domain

Released:
DOI: 10.2210/pdb4fof/pdb
PDB entry 4fof coloured by chain, front view.
PDB entry 4fof coloured by chain, side view.
PDB entry 4fof coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
A photo-labile thioether linkage to phycoviolobilin provides the foundation for the blue/green photocycles in DXCF-cyanobacteriochromes.
Burgie ES, Walker JM, Phillips GN, Vierstra RD
Structure 21 88-97 (2013)
PMID: 23219880

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.6 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DLC7 (Residues: 430-591; Coverage: 17%)
Gene name: tll0569
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
Ligand VRB 1 x VRB
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: R32
Unit cell:
a: 104.232Å b: 104.232Å c: 115.314Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.18 0.229
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Phytochromes: an atomic perspective on photoactivation and signaling.
Burgie et al. (2014)
6 more

2 mentions without citation

Photoreceptor engineering.
Ziegler et al. (2015)
1 more