› 4fou

X-ray diffraction
2.1Å resolution

Structure of the PilZ-FimX(EAL domain)-c-di-GMP complex responsible for the regulation of bacterial Type IV pilus biogenesis

Released:
DOI: 10.2210/pdb4fou/pdb
PDB entry 4fou coloured by chain, front view.
PDB entry 4fou coloured by chain, side view.
PDB entry 4fou coloured by chain, top view.
Source organism: Xanthomonas citri pv. citri str. 306
Primary publication:
Structure of the PilZ-FimXEAL-c-di-GMP Complex Responsible for the Regulation of Bacterial Type IV Pilus Biogenesis.
Guzzo CR, Dunger G, Salinas RK, Farah CS
J Mol Biol 425 2174-97 (2013)
PMID: 23507310

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241368 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FimX Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.61 KDa
Source organism: Xanthomonas citri pv. citri str. 306
Expression system: Escherichia coli BL21(DE3)
Structure domains: EAL domain
Type IV fimbriae assembly protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 117 amino acids
Theoretical weight: 12.44 KDa
Source organism: Xanthomonas citri pv. citri str. 306
Expression system: Escherichia coli BL21(DE3)
Structure domains: predicted glycosyltransferase like domains

Ligands and Environments

3 bound ligands:
Ligand C2E 2 x C2E
Ligand CA 3 x CA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P65
Unit cell:
a: 126.787Å b: 126.787Å c: 107.317Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Biofilms and Cyclic di-GMP (c-di-GMP) Signaling: Lessons from Pseudomonas aeruginosa and Other Bacteria.
Valentini et al. (2016)
8 more

4 mentions without citation

The World of Cyclic Dinucleotides in Bacterial Behavior.
Aline Dias da et al. (2020)
3 more