› 4fp9

X-ray diffraction
2.9Å resolution

Human MTERF4-NSUN4 protein complex

Released:
DOI: 10.2210/pdb4fp9/pdb
PDB entry 4fp9 coloured by chain, front view.
PDB entry 4fp9 coloured by chain, side view.
PDB entry 4fp9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis.
Spåhr H, Habermann B, Gustafsson CM, Larsson NG, Hallberg BM
Proc Natl Acad Sci U S A 109 15253-8 (2012)
PMID: 22949673

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182298 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
5-methylcytosine rRNA methyltransferase NSUN4 Chains: A, C, D, F
Molecule details ›
Chains: A, C, D, F
Length: 360 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CB9 (Residues: 26-384; Coverage: 94%)
Gene name: NSUN4
Sequence domains: 16S rRNA methyltransferase RsmB/F
Structure domains:
mTERF domain-containing protein 2 processed Chains: B, E, G, H
Molecule details ›
Chains: B, E, G, H
Length: 335 amino acids
Theoretical weight: 38.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6M4 (Residues: 47-381; Coverage: 88%)
Gene names: HSPC096, MTERF4, MTERFD2
Sequence domains: mTERF

Ligands and Environments

2 bound ligands:
Ligand SAM 4 x SAM
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 78.76Å b: 82.27Å c: 507.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.246
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Maintenance and Expression of Mammalian Mitochondrial DNA.
Gustafsson et al. (2016)
23 more

4 mentions without citation

Structural biology and chemistry of protein arginine methyltransferases.
Schapira et al. (2014)
3 more