› 4fpi

X-ray diffraction
2.2Å resolution

Crystal Structure of 5-chloromuconolactone isomerase from Rhodococcus opacus 1CP

Released:
DOI: 10.2210/pdb4fpi/pdb
PDB entry 4fpi coloured by chain, front view.
PDB entry 4fpi coloured by chain, side view.
PDB entry 4fpi coloured by chain, top view.
Source organism: Rhodococcus opacus
Primary publication:
X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP.
Ferraroni M, Kolomytseva M, Golovleva LA, Scozzafava A
J Struct Biol 182 44-50 (2013)
PMID: 23376735

Function and Biology Details

Reaction catalysed: 
(+)-muconolactone = 5-oxo-4,5-dihydrofuran-2-acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Muconolactone Delta-isomerase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, R, S, T, U
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, R, S, T, U
Length: 94 amino acids
Theoretical weight: 11.2 KDa
Source organism: Rhodococcus opacus
UniProt:
  • Canonical: Q8G9L0 (Residues: 1-94; Coverage: 100%)
Gene names: R1CP_39625, clcF
Sequence domains: Muconolactone delta-isomerase
Structure domains: Dimeric alpha+beta barrel

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 122.187Å b: 79.657Å c: 125.907Å
α: 90° β: 105.54° γ: 90°
R-values:
R R work R free
0.232 0.228 0.301

Quick links

Citations

1 review citation

Substrate, product, and cofactor: The extraordinarily flexible relationship between the CDE superfamily and heme.
Celis et al. (2015)
1 other article