› 4fqr

X-ray diffraction
4.1Å resolution

Crystal structure of broadly neutralizing antibody C05 bound to H3 influenza hemagglutinin

Released:
DOI: 10.2210/pdb4fqr/pdb
PDB entry 4fqr coloured by chain, front view.
PDB entry 4fqr coloured by chain, side view.
PDB entry 4fqr coloured by chain, top view.
Source organisms:
Primary publication:
Cross-neutralization of influenza A viruses mediated by a single antibody loop.
Ekiert DC, Kashyap AK, Steel J, Rubrum A, Bhabha G, Khayat R, Lee JH, Dillon MA, O'Neil RE, Faynboym AM, Horowitz M, Horowitz L, Ward AB, Palese P, Webby R, Lerner RA, Bhatt RR, Wilson IA
Nature 489 526-32 (2012)
PMID: 22982990

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212657 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin HA1 chain Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Length: 323 amino acids
Theoretical weight: 35.51 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 27-345; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 174 amino acids
Theoretical weight: 20.04 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 346-519; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Broadly neutralizing antibody C05, heavy chain Chains: a, c, e, g, i, k, m, o, q, s, u, w
Molecule details ›
Chains: a, c, e, g, i, k, m, o, q, s, u, w
Length: 241 amino acids
Theoretical weight: 25.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Broadly neutralizing antibody C05, light chain Chains: b, d, f, h, j, l, n, p, r, t, v, x
Molecule details ›
Chains: b, d, f, h, j, l, n, p, r, t, v, x
Length: 214 amino acids
Theoretical weight: 23.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 30 x NAG
1 modified residue:
Ligand PCA 12 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 142.02Å b: 158.62Å c: 178.5Å
α: 89.95° β: 85.44° γ: 84.37°
R-values:
R R work R free
0.31 0.309 0.334
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

89 review citations

Advances in the development of influenza virus vaccines.
Krammer et al. (2015)
88 more

11 mentions without citation

Broadly neutralizing antibodies against influenza viruses.
Laursen et al. (2013)
10 more