› 4frn

X-ray diffraction
3.43Å resolution

Crystal structure of the cobalamin riboswitch regulatory element

Released:
DOI: 10.2210/pdb4frn/pdb
PDB entry 4frn coloured by chain, front view.
PDB entry 4frn coloured by chain, side view.
PDB entry 4frn coloured by chain, top view.
Source organism: marine metagenome
Primary publication:
B12 cofactors directly stabilize an mRNA regulatory switch.
Johnson JE, Reyes FE, Polaski JT, Batey RT
Nature 492 133-7 (2012)
PMID: 23064232

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195904 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Cobalamin riboswitch aptamer domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 nucleotides
Theoretical weight: 32.91 KDa
Source organism: marine metagenome
Expression system: Not provided
Sequence domains: AdoCbl variant RNA

Ligands and Environments

2 bound ligands:
Ligand I2A 2 x I2A
Ligand BA 12 x BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 55.605Å b: 81.833Å c: 147.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.251 0.273
Expression system: Not provided

Quick links

Citations

24 review citations

A decade of riboswitches.
Serganov et al. (2013)
23 more

14 mentions without citation

Long-Range Interactions in Riboswitch Control of Gene Expression.
Jones et al. (2017)
13 more