› 4fu4

X-ray diffraction
2.85Å resolution

Human collagenase 3 (MMP-13) with peptide from pro-domain

Released:
DOI: 10.2210/pdb4fu4/pdb
PDB entry 4fu4 coloured by chain, front view.
PDB entry 4fu4 coloured by chain, side view.
PDB entry 4fu4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of full-length human collagenase 3 (MMP-13) with peptides in the active site defines exosites in the catalytic domain.
Stura EA, Visse R, Cuniasse P, Dive V, Nagase H
FASEB J 27 4395-405 (2013)
PMID: 23913860

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155287 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Collagenase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 42.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45452 (Residues: 104-471; Coverage: 81%)
Gene name: MMP13
Sequence domains:
Structure domains:
Collagenase 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 26 amino acids
Theoretical weight: 3.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45452 (Residues: 25-50; Coverage: 6%)
Gene name: MMP13
Sequence domains: Putative peptidoglycan binding domain

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 8 x CA
Ligand CL 4 x CL
Ligand EDO 26 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 127.08Å b: 156.58Å c: 106.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.247
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Matrix metalloproteinases as breast cancer drivers and therapeutic targets.
Radisky et al. (2015)
3 more

7 mentions without citation

Matrix metalloproteinase interactions with collagen and elastin.
Van Doren SR. (2015)
6 more