› 4fum

X-ray diffraction
2.4Å resolution

Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms

Released:
DOI: 10.2210/pdb4fum/pdb
PDB entry 4fum coloured by chain, front view.
PDB entry 4fum coloured by chain, side view.
PDB entry 4fum coloured by chain, top view.
Source organism: Staphylococcus epidermidis RP62A
Primary publication:
Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms.
Conrady DG, Wilson JJ, Herr AB
Proc Natl Acad Sci U S A 110 E202-11 (2013)
PMID: 23277549

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Accumulation associated protein Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.57 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HKE8 (Residues: 2017-2223; Coverage: 9%)
Gene names: SERP2398, aap
Sequence domains:
Structure domains: Resuscitation-promoting factor rpfb.

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SCN 1 x SCN
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 89.721Å b: 33.637Å c: 81.325Å
α: 90° β: 121.06° γ: 90°
R-values:
R R work R free
0.201 0.198 0.255
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus.
Foster et al. (2014)
18 more

2 mentions without citation

Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms.
Conrady et al. (2013)
1 more