› 4fus

X-ray diffraction
1.75Å resolution

The X-ray structure of Hahella chejuensis family 48 glycosyl hydrolase

Released:
DOI: 10.2210/pdb4fus/pdb
PDB entry 4fus coloured by chain, front view.
PDB entry 4fus coloured by chain, side view.
PDB entry 4fus coloured by chain, top view.
Source organism: Hahella chejuensis KCTC 2396
Primary publication:
Sequence, structure, and evolution of cellulases in glycoside hydrolase family 48.
Sukharnikov LO, Alahuhta M, Brunecky R, Upadhyay A, Himmel ME, Lunin VV, Zhulin IB
J Biol Chem 287 41068-77 (2012)
PMID: 23055526

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CBM2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 695 amino acids
Theoretical weight: 77.91 KDa
Source organism: Hahella chejuensis KCTC 2396
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2SJA2 (Residues: 33-704; Coverage: 73%)
Gene name: HCH_02465
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
7 bound ligands:
Ligand PEG 1 x PEG
Ligand GOL 5 x GOL
Ligand PO4 2 x PO4
Ligand ACT 4 x ACT
Ligand EDO 12 x EDO
Ligand CA 1 x CA
Ligand NA 2 x NA
1 modified residue:
Ligand OHI 1 x OHI

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 61.69Å b: 78.389Å c: 67.075Å
α: 90° β: 98.94° γ: 90°
R-values:
R R work R free
0.156 0.154 0.205
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Experimental and computational studies of cellulases as bioethanol enzymes.
Ranganathan et al. (2022)
14 other articles

2 mentions without citation

Sequence, structure, and evolution of cellulases in glycoside hydrolase family 48.
Sukharnikov et al. (2012)
1 more