› 4fw1

X-ray diffraction
1.86Å resolution

Crystal structure of two-domain RSV INTEGRASE covalently linked with DNA

Released:
DOI: 10.2210/pdb4fw1/pdb
PDB entry 4fw1 coloured by chain, front view.
PDB entry 4fw1 coloured by chain, side view.
PDB entry 4fw1 coloured by chain, top view.
Source organism: Rous sarcoma virus - Prague C
Primary publication:
A possible role for the asymmetric C-terminal domain dimer of Rous sarcoma virus integrase in viral DNA binding.
Shi K, Pandey KK, Bera S, Vora AC, Grandgenett DP, Aihara H
PLoS One 8 e56892 (2013)
PMID: 23451105

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.75 KDa
Source organism: Rous sarcoma virus - Prague C
Expression system: Escherichia coli
UniProt:
  • Canonical: P03354 (Residues: 1329-1550; Coverage: 14%)
Gene name: gag-pol
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 54.8Å b: 67.48Å c: 126.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Retroviral Integrase: Then and Now.
Andrake et al. (2015)
2 more

2 mentions without citation

Crystal structure of the Rous sarcoma virus intasome.
Yin et al. (2016)
1 more