› 4fw2

X-ray diffraction
2.65Å resolution

Crystal structure of RSV three-domain integrase with disordered N-terminal domain

Released:
DOI: 10.2210/pdb4fw2/pdb
PDB entry 4fw2 coloured by chain, front view.
PDB entry 4fw2 coloured by chain, side view.
PDB entry 4fw2 coloured by chain, top view.
Source organism: Rous sarcoma virus - Prague C
Primary publication:
A possible role for the asymmetric C-terminal domain dimer of Rous sarcoma virus integrase in viral DNA binding.
Shi K, Pandey KK, Bera S, Vora AC, Grandgenett DP, Aihara H
PLoS One 8 e56892 (2013)
PMID: 23451105

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 29.93 KDa
Source organism: Rous sarcoma virus - Prague C
Expression system: Escherichia coli
UniProt:
  • Canonical: P03354 (Residues: 1281-1550; Coverage: 17%)
Gene name: gag-pol
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 176.4Å b: 56.168Å c: 65.276Å
α: 90° β: 97.86° γ: 90°
R-values:
R R work R free
0.181 0.174 0.244
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Retroviral Integrase: Then and Now.
Andrake et al. (2015)
2 more

1 mention without citation

A possible role for the asymmetric C-terminal domain dimer of Rous sarcoma virus integrase in viral DNA binding.
Shi et al. (2013)