› 4fxb

X-ray diffraction
2.9Å resolution

Crystal structure of CYP105N1 from Streptomyces coelicolor: a cytochrome P450 oxidase in the coelibactin siderophore biosynthetic pathway

Released:
DOI: 10.2210/pdb4fxb/pdb
PDB entry 4fxb coloured by chain, front view.
PDB entry 4fxb coloured by chain, side view.
PDB entry 4fxb coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Crystal structure of cytochrome P450 CYP105N1 from Streptomyces coelicolor, an oxidase in the coelibactin siderophore biosynthetic pathway.
Lim YR, Hong MK, Kim JK, Doan TT, Kim DH, Yun CH, Chun YJ, Kang LW, Kim D
Arch Biochem Biophys 528 111-7 (2012)
PMID: 23000034

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 45.98 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EWP1 (Residues: 1-411; Coverage: 100%)
Gene names: SC4C2.21, SCO7686
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P6122
Unit cell:
a: 133.086Å b: 133.086Å c: 227.111Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.282 0.281 0.302
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Beyond iron: non-classical biological functions of bacterial siderophores.
Johnstone et al. (2015)
11 other articles

2 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
1 more